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From the texture of the membrane, the most important indicator is the amount of protein that can be bound per unit area. The binding capacity of the nitrocellulose membrane is mainly related to the purity of the nitrocellulose of the membrane. Some nitrocellulose membranes on the market usually have a large amount of cellulose acetate, thereby reducing the amount of protein binding. If 100% pure nitrocellulose is used, the maximum protein binding is guaranteed up to 80-150 μg/cm2. Sartorius' NC membrane is a dense 100% nitrocellulose filter that is an excellent filter for Western, Northern and Southern hybridization. Due to the 100% purity, the non-specific binding is also greatly reduced, the hybridization background is reduced, and the maximum protein binding is ensured, up to 150-200 μg / cm 2 . In addition, Sartorius's NC film is excellent in quality and toughness and strength. Compared with the traditional NC membrane, the texture is brittle, and the rinsing will be damaged once or twice. The advantages of Sartorius' NC membrane are highlighted. It can be used repeatedly and is not easy to break. It is the best choice for Western, Northern and Southern hybridization experiments. .
From the viewpoint of the selection of the membrane, depending on the molecular weight of the protein to be transferred, a nitrocellulose membrane having a different pore diameter is selected. Because as the membrane pore size continues to decrease, the membrane binds to low molecular weight proteins more firmly. However, if the membrane pore size is less than 0.1 mm, protein transfer is difficult. Therefore, we usually use nitrocellulose membranes of both 0.45 μm and 0.2 μm sizes. A protein larger than 20 kD can be used with a 0.45 μm membrane, and a protein smaller than 20 kD is a 0.2 μm membrane. If a membrane of 0.45 μm is used, a "Blowthrough" phenomenon occurs. Sartorius's NC film is also available in two options, covering almost all users' needs. A nitrocellulose membrane with a pore size of 0.2 μm is recommended for transfer of small molecular proteins and nucleic acids to avoid sample loss due to membrane transfer; nitrocellulose membranes with a pore size of 0.45 μm are recommended for transfer of macromolecular proteins and nucleic acids As well as most analytical blots, including protein ssDNA and RNA transfer.
From the point of view of the use of the film, the NC film on the market needs to be pretreated to be used, and there is also a need for pretreatment which can be used directly. Sartorius' NC film does not need to be pretreated with anhydrous methanol, and the membrane only needs to be simply wetted in water and then placed in transfer buffer. Simple operation can be used with confidence.
From now on, log on to the online feedback page of the official website of Sartorius and apply for trial. Leave your trial number and you can get any of the Sartorius nitrocellulose hybrid membranes (item number: NC-0.22μm or NC-0.45μm). Trial installation, limited to the top 300, first come first served. The trial will be issued the second week of receiving your email application. For more information, please call: 400.920.9889 | 800.820.9889
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