The cell cycle is an important test parameter, and the impact on cell cycle changes plays an important role in tumor development and drug development. For example, compounds known to inhibit mitosis are mostly used to slow the growth of tumor cells.
Two phases in the cell cycle are most important: G1 to S and G2 to M; these two phases are in the period of complex and active molecular level changes, which are easily affected by environmental conditions. If they can be artificially regulated, they will be It is important to understand the growth and development of organisms and control tumor growth. If the cell cycle changes can be detected easily and effectively, it will be of great significance for drug development and disease research.
The relationship between FUCCI cell cycle markers and cell cycle changes. The expression of Cdt1-RFP red is G1 phase, Geminin-GFP green is expressed as G2/M phase, and Cdt1-RFP red and Geminin-GFP green are expressed as S phase.
Methods for measuring the cell cycle are:
â—Isotopic labeling method â—Flow cytometry method â—Fluorescence detection method based on cell imaging
The "Cell Cycle Detection Method" manual we have compiled details the common detection methods and principles. The MD-based MiniMax system and the high-content imaging analysis system based on imaging high-throughput detection methods can be combined with 2D and 3D. Detection means more accurately simulate the biological status of cancer cells in vivo and effectively evaluate new anti-cancer strategies.
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