High-performance liquid-post-column derivatization-fluorescence detection method for the determination of aflatoxin-琛航科技

Determination of aflatoxin by high performance liquid phase - post - column derivatization - fluorescence detection

琛航科技测试 report

A new generation of post-column derivatization system with high sensitivity and lower price.

The CoMetro 6000 series post-column derivatization system (post-column derivatization) pioneered new standards for performance, price and shape of post-column derivatization systems. The unique reactor design creates a new generation of post-column derivatization systems— the high performance and low price of the 6000 Series post-column derivatization system (post-column derivatization) .

CoMetro 6000 Series Post-column Derivative System (post-column derivative) Features:

Ø     Fast and easy to set up

Simply connect an inlet from the column and an outlet into the detector for immediate installation.

Ø     Modular design

Optional single or double reaction core. The heating reaction can be carried out simultaneously, and the two-step reaction process of the normal temperature reaction.

Optional 1 , 2 or more derivatizing pumps.

Ø   Reduces joint and pipe connections to prevent leakage.

Ø   All connectors are located on the front panel for user convenience.

Ø   Panel display for easy setup

The front panel of the reactor shows the set and actual temperatures of each reactor.

The front panel of the derived pump shows the flow rate, pressure and pressure limits for each reagent.

Ø   External control

The flow rate of the pump and the temperature of the reactor can be controlled via the front panel or externally via RS232 .

Ø   The modular reactor allows the reactor core to be replaced with just 2 connectors.

Ø   The newly designed reaction core greatly reduces the peak spread.

Ø   The system is full of PEEK flow paths.  

First, the experimental conditions

Cometro High Performance Liquid Chromatography System;

Cometro (United States Connaught) 6000 LDI Pump ;

Fluorescence detector with excitation wavelength of 360 nm and emission wavelength of 450 nm ;

Cometro (Connolly U.S.) system, post-column derivatization, post-column derivatization 70 degrees, the derivatizing agent: 0.03% aqueous solution of iodine in methanol,

The flow rate is 0.3 ml/min ;

Scienhome (Tianjin Haohang ) Kromasil c18 250*4.6mm column;

Mobile phase: methanol - acetonitrile - water = 40:10:50 ;

Flow rate: 0.8 ml/min ;

Sample: aflatoxin stock solution;

Injection volume: 10ul .

Second, the experimental results

Third, the results of the discussion

The above results show baseline noise ( 0.2mv ), sensitivity, signal-to-noise ratio ( >10 ), efficiency ( >9000 ), and resolution between main peaks ( >1.5 ).