Sanyo carbon dioxide incubator is an indispensable equipment for scientific research in modern large and medium-sized hospitals, and is distributed in various laboratories of hospitals. It is a device for in vitro culture of tissues, cells and bacteria by simulating a growth environment similar to the tissue in the living body in an incubator.
Sanyo CO2 incubator is ready for use before use
1. The carbon dioxide incubator should be used by a special person. Do not twist any switch and adjustment knob on the operation panel once it is fixed, so as not to affect the temperature, carbon dioxide concentration and humidity fluctuations in the box, and reduce the sensitivity of the machine.
2, the box should be placed horizontally, avoid direct sunlight and dust, etc., to ensure that the airflow around the instrument, the rear and side should leave a gap of 5cm.
3. Install a regulated power supply to ensure that the power supply voltage fluctuation does not exceed 220V ± 10%.
4. The water to be added must be distilled water or non-ionized water to prevent the mineral deposits from causing corrosion in the water tank. Check whether the water in the tank is sufficient. The water level should be added until the “water level indicator light†is turned off.
5, use the disinfection of alcohol or iodine solution in the front box to thoroughly scrub to prevent other microbial contamination, resulting in experimental failure.
6. Install air conditioner in the room to keep the ambient temperature at around 25 °C.
7. The carbon dioxide used must be pure, otherwise the sensitivity of the carbon dioxide sensor and the contamination of the carbon dioxide filter unit are reduced.
Sanyo carbon dioxide sensor is properly inflated
The pressure in the carbon dioxide incubator is controlled by a carbon dioxide gas sensor. The normal feedback of the CO2 sensor requires an intake pressure of 0.06 MPa. When the air pressure in the box is lowered, the carbon dioxide sensor is rhythmically opened and closed under low pressure stimulation, and is slowly inflated into the tank. When the intake pressure reaches 0.1 MPa, the adjustment is automatically stopped.
If the inflation method is improper, the intake pressure will increase, and when it exceeds 0.1MPa or even 0.2MPa, the carbon dioxide sensor will be damaged. At the beginning, this damage effect is not obvious, it is not easy to detect, repeated error operation, and the carbon dioxide sensor damage accumulation eventually causes fatal damage, which makes the air pressure adjustment malfunction.
Therefore, the life of the carbon dioxide sensor is related to the size of the carbon dioxide intake pressure, and the performance of the carbon dioxide sensor can be protected by adjusting the intake pressure. Carbon dioxide sensors are expensive and difficult to replace, so be sure to adjust the intake pressure to extend the life of the carbon dioxide sensor.
Carbon dioxide incubator humidity
The humidity inside the box is a very important but often overlooked factor for the training work. Because the saturated humidity environment can avoid the escape of carbon dioxide in the culture solution, the pH value in the culture solution is stable, which is good for cell growth, sufficient humidity level and fast enough humidity recovery speed (such as after opening and closing the door) to ensure that it will not be excessive. Drying causes the culture to fail.
Therefore, during the use of the carbon dioxide incubator, a certain amount of sterilized water (3/2 to full plate) is placed in the tray inside the box to make the inside of the box a saturated humidity environment. If the user forgets to add water, the cultured cells are in a low humidity or even dry heat environment, the carbon dioxide gas in the culture liquid is volatile, the pH value of the liquid rises, affecting cell growth, and efforts should be made to avoid this problem in the work.
When the sample is used for a long time, the box is stored in the compartment, and the culture is stored in a basket or a porcelain plate, so that the access is fast, the opening time is shortened, the gas and humidity in the box are changed, the gas is saved, and the cell growth environment is stabilized.
Disinfection in Sanyo Carbon Dioxide Incubator
Carbon dioxide incubator contamination is a major factor leading to cell culture failure, so the main way to reduce and prevent pollution is to minimize the areas and surfaces where microorganisms can grow and to take disinfection measures.
For example, for the problem of bacteria and bacteria in the hot and humid environment in the box, add 2mL of new Jieer liquid in 1000mL water, regularly disinfect the air in the box with alcohol and ultraviolet light, and wipe the culture alcohol into the box. The water in the plate should be changed at least once a week. Control pollution, maintain the adaptive evaporation, reduce the rate of microbial growth in the box.
In order to protect the tank from contamination and to ensure bio-cleanability in the instrument cabinet, some carbon dioxide incubators are designed with automatic decontamination devices to effectively prevent contamination. There are a variety of sterilization methods, such as CO2 incubators with UV disinfection.
Other designs and production of HEPA filters can filter the air in the incubator, can filter and remove 99.97% of particles above 0.3μm, and also designed to enable the environment to reach high temperature and heat inside the box to kill contaminated microorganisms. Incubator for sterilization purposes.
Sanyo carbon dioxide incubator zero drift
During the use of the incubator, the carbon dioxide zero will naturally drift, this phenomenon can not be expressed in the box gas display box, the user is not easy to notice the drift results. Whether the zero point of carbon dioxide drifts, the carbon dioxide gas tester can be used to collect the gas in the incubator from the sampling port on the control panel, and then the carbon dioxide concentration can be measured by the gas chromatograph.
The step of adjusting the zero point of the carbon dioxide incubator (usually zeroing with air as the standard, once every six months.):
1. Set the carbon dioxide concentration setting to zero in order to prevent carbon dioxide from entering the tank.
2. Open all the doors for 5 minutes, add water to the basin, close all the doors, and stabilize for more than 12 hours.
3. After the isothermal humidity is fully stabilized, observe whether the carbon dioxide concentration display value is zero. If it is not zero, correct it to zero (press the Mode button to the Cal file, press the left button or the right button to select “CO2 CAL XX.Xâ€). Press the up or down button to adjust the value to zero, then press Enter to confirm).
4. Reset the carbon dioxide concentration setting back (eg 5.0%).
Sanyo CO2 incubator is ready for use before use
1. The carbon dioxide incubator should be used by a special person. Do not twist any switch and adjustment knob on the operation panel once it is fixed, so as not to affect the temperature, carbon dioxide concentration and humidity fluctuations in the box, and reduce the sensitivity of the machine.
2, the box should be placed horizontally, avoid direct sunlight and dust, etc., to ensure that the airflow around the instrument, the rear and side should leave a gap of 5cm.
3. Install a regulated power supply to ensure that the power supply voltage fluctuation does not exceed 220V ± 10%.
4. The water to be added must be distilled water or non-ionized water to prevent the mineral deposits from causing corrosion in the water tank. Check whether the water in the tank is sufficient. The water level should be added until the “water level indicator light†is turned off.
5, use the disinfection of alcohol or iodine solution in the front box to thoroughly scrub to prevent other microbial contamination, resulting in experimental failure.
6. Install air conditioner in the room to keep the ambient temperature at around 25 °C.
7. The carbon dioxide used must be pure, otherwise the sensitivity of the carbon dioxide sensor and the contamination of the carbon dioxide filter unit are reduced.
Sanyo carbon dioxide sensor is properly inflated
The pressure in the carbon dioxide incubator is controlled by a carbon dioxide gas sensor. The normal feedback of the CO2 sensor requires an intake pressure of 0.06 MPa. When the air pressure in the box is lowered, the carbon dioxide sensor is rhythmically opened and closed under low pressure stimulation, and is slowly inflated into the tank. When the intake pressure reaches 0.1 MPa, the adjustment is automatically stopped.
If the inflation method is improper, the intake pressure will increase, and when it exceeds 0.1MPa or even 0.2MPa, the carbon dioxide sensor will be damaged. At the beginning, this damage effect is not obvious, it is not easy to detect, repeated error operation, and the carbon dioxide sensor damage accumulation eventually causes fatal damage, which makes the air pressure adjustment malfunction.
Therefore, the life of the carbon dioxide sensor is related to the size of the carbon dioxide intake pressure, and the performance of the carbon dioxide sensor can be protected by adjusting the intake pressure. Carbon dioxide sensors are expensive and difficult to replace, so be sure to adjust the intake pressure to extend the life of the carbon dioxide sensor.
Carbon dioxide incubator humidity
The humidity inside the box is a very important but often overlooked factor for the training work. Because the saturated humidity environment can avoid the escape of carbon dioxide in the culture solution, the pH value in the culture solution is stable, which is good for cell growth, sufficient humidity level and fast enough humidity recovery speed (such as after opening and closing the door) to ensure that it will not be excessive. Drying causes the culture to fail.
Therefore, during the use of the carbon dioxide incubator, a certain amount of sterilized water (3/2 to full plate) is placed in the tray inside the box to make the inside of the box a saturated humidity environment. If the user forgets to add water, the cultured cells are in a low humidity or even dry heat environment, the carbon dioxide gas in the culture liquid is volatile, the pH value of the liquid rises, affecting cell growth, and efforts should be made to avoid this problem in the work.
When the sample is used for a long time, the box is stored in the compartment, and the culture is stored in a basket or a porcelain plate, so that the access is fast, the opening time is shortened, the gas and humidity in the box are changed, the gas is saved, and the cell growth environment is stabilized.
Disinfection in Sanyo Carbon Dioxide Incubator
Carbon dioxide incubator contamination is a major factor leading to cell culture failure, so the main way to reduce and prevent pollution is to minimize the areas and surfaces where microorganisms can grow and to take disinfection measures.
For example, for the problem of bacteria and bacteria in the hot and humid environment in the box, add 2mL of new Jieer liquid in 1000mL water, regularly disinfect the air in the box with alcohol and ultraviolet light, and wipe the culture alcohol into the box. The water in the plate should be changed at least once a week. Control pollution, maintain the adaptive evaporation, reduce the rate of microbial growth in the box.
In order to protect the tank from contamination and to ensure bio-cleanability in the instrument cabinet, some carbon dioxide incubators are designed with automatic decontamination devices to effectively prevent contamination. There are a variety of sterilization methods, such as CO2 incubators with UV disinfection.
Other designs and production of HEPA filters can filter the air in the incubator, can filter and remove 99.97% of particles above 0.3μm, and also designed to enable the environment to reach high temperature and heat inside the box to kill contaminated microorganisms. Incubator for sterilization purposes.
Sanyo carbon dioxide incubator zero drift
During the use of the incubator, the carbon dioxide zero will naturally drift, this phenomenon can not be expressed in the box gas display box, the user is not easy to notice the drift results. Whether the zero point of carbon dioxide drifts, the carbon dioxide gas tester can be used to collect the gas in the incubator from the sampling port on the control panel, and then the carbon dioxide concentration can be measured by the gas chromatograph.
The step of adjusting the zero point of the carbon dioxide incubator (usually zeroing with air as the standard, once every six months.):
1. Set the carbon dioxide concentration setting to zero in order to prevent carbon dioxide from entering the tank.
2. Open all the doors for 5 minutes, add water to the basin, close all the doors, and stabilize for more than 12 hours.
3. After the isothermal humidity is fully stabilized, observe whether the carbon dioxide concentration display value is zero. If it is not zero, correct it to zero (press the Mode button to the Cal file, press the left button or the right button to select “CO2 CAL XX.Xâ€). Press the up or down button to adjust the value to zero, then press Enter to confirm).
4. Reset the carbon dioxide concentration setting back (eg 5.0%).
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