When microscopic examination revealed that the rotifers had orange-red resting eggs, the rotifers could be placed under conditions of low salinity (9 or 20 ‰) to make the rotifers form dormant eggs. It can also be stimulated by temperature, that is, the rotifer cultured in a low temperature condition is placed in a culture solution at 29-30°C. This is repeated every two days and can also artificially form a large number of care eggs for fertilization. Under normal circumstances, the rotifers naturally reproduce. When they reach a higher density and the food consumes light, a large number of dormant eggs emerge, and the dormant eggs are harvested. They can be screened out with a fine mesh of 85 microns, and then the residue is removed and a little air-dried. Put it into the bottle and store it in a wax seal. It can also be condensed with the body of water and stored directly in the refrigerator (4°C) for years. The rotifer dormant eggs preserved for 1 year at 3°C ​​were placed in natural seawater (salinity 32‰, water temperature 10-1818°C) and eggs could be successively 3-4 days.