Annexin V and the indissoluble bond of apoptosis

Apoptosis or programmed cell death refers to an advanced regulatory process in which cells remove unwanted and unfunctioning cells. Apoptosis can occur in cell growth, aging, immune responses, or when cells are damaged by disease and toxic factors, such as radiopharmaceuticals and chemotherapy.

Apoptotic cells produce many important changes, including cell shrinkage, cytoplasmic agglutination, DNA fragmentation and bubble formation, and lead to the separation of cell components to form apoptotic bodies, which are then followed by macrophages or thin walls. Tissue cells are swallowed. During early apoptosis, phosphatidylserine (PS), which is only distributed inside the lipid bilayer of normal cell membranes, turns from the inside of the lipid membrane to the outside, while Annexin V acts as a protein with high affinity for PS. The labeling of fluorescein (FITC, Cy3, PE, etc.) can conveniently indicate the occurrence of apoptosis, thereby conducting research on apoptosis. It is precisely because of this characteristic of AnnexinV that it has become an evergreen tree in the field of apoptosis research!

As a professional solution provider in the field of apoptosis, Amy Jie recommends the Biovision brand of Annexin V apoptosis assay kit, which contains various Annexin V derivatives and a variety of fluorescent conjugates to meet the different needs of customers. , fuel your apoptosis research!

The Annexin V-FITC Apoptosis Kit Plus (K201) is based on the phenomenon that the phosphoryl arginine (PS) on the inner surface of the plasma membrane is transferred to the cell surface shortly after the initiation of apoptosis. This one-step dyeing technique takes only ten minutes. At the same time, the detection method can be directly used for living cells without fixing. Annexin V-FITC Apoptosis Detection Kit Plus has Annexin V-FITC, SYTOX green dye has been combined with buffer. SYTOX green dyes are unable to penetrate living cells and apoptotic cells, but can stain necrotic cells and bind to the intracellular nucleic acids to emit intense green fluorescence. After staining a cell population with Annexin V-FITC and SYTOX green dye in our buffer, apoptotic cells emit green fluorescence, dead cells emit higher levels of green fluorescence, and live cells have little or no fluorescence. The stained cell population can be analyzed by flow cytometry with argon excitation at 488 nm. Both Annexin V-FITC and SYTOX green dyes emit green fluorescence, which can be detected in the FL1 channel. If additional probes are added for the colorful labeling experiment, other channels can be detected.

Annexin V 与细胞凋亡的不解之缘Annexin V 与细胞凋亡的不解之缘

The BioVision Apoptosis Cell Isolation Kit (K258-30) provides an easy and efficient way to enrich for apoptotic cells by binding to Vinxin V in magnetic beads (MagBeads), combined with magnetic beads Dead cells are enriched by magnetic force, while non-apoptotic cells are still free. Isolated apoptotic cells and healthy cells can be used for the study of various apoptotic mechanisms and pathways. This kit can also be used to remove dead cells during cell culture. At the same time, the operation is fast and convenient, the steps are simple, and it takes only about one hour to complete the test.

In addition to the kits above, Biovision offers a complete range of Annexin V-label reagents, proteins, reaction buffers, antibodies, etc., to provide customers with a full range of Annexin V-cell apoptosis studies.

Amy Jie is also recommended for you. Annexin V antibody (3357-100) is a rabbit polyclonal antibody that can be used in immunocyto A variety of applications.

Annexin V 与细胞凋亡的不解之缘

Amy Jie finds and provides the best product solutions for you. BioVision has an outstanding influence in the development and research of apoptosis detection and metabolism detection. Including a variety of detection and analysis kits, related antibodies, recombinant proteins and cytokines and enzymes, as well as other innovative research tools. Its products cover areas such as apoptosis research, energy metabolism, cell/tissue protein extraction, oxidative stress and injury, metabolism and obesity, cell proliferation and toxicity, signal transduction, and stem cell research. As the general agent of many well-known brands in China and the regional agent in China, Aimei and BioVision together provide researchers with the best and latest cell biology research tools to continuously expand the quality of products and services. If you are interested in the above products, please call the free customer line 400-6800-868 to contact Aimei Technology Co., Ltd. for product information and complete experimental solutions.

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Throat Pain Relief Patch
[Name] Medical Cold Patch
[Package Dimension] 36 round pieces
The pain relief patch is composed of three layers, namely, backing lining, middle gel and protective film. It is free from pharmacological, immunological or metabolic ingredients.
[Scope of Application] For cold physiotherapy, closed soft tissue only.
[Indications]
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[How To Use a Patch]
Please follow the Schematic Diagram. One piece, one time.
The curing effect of each piece can last for 6-8 hours.
[Attention]
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[Storage Conditions] Store below 30c in a dry place away from heat and direct sunlight.







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