Poly ADP ribose polymerase (PARP) is a sensor of DNA damage that plays a major role in DNA repair and maintenance of genomic stability. The PARP enzymes that can repair DNA are all discoveries of modern genetics. PARP is a cleavage substrate for caspase, a core member of apoptosis. Therefore, it plays an important role in DNA damage repair and apoptosis.
When DNA is damaged, PARP can catalyze the polymerization of poly ADP ribose to itself and neighboring histones in a NAD+-dependent manner, thereby participating in a series of events in DNA base excision repair. When the intracellular NAD+ pool is largely eliminated, it will cause PARP-mediated necrosis induction, in which PARP-1 cleavage promotes apoptosis by preventing DNA repair and blocking the supply of necrotic energy. Experiments have shown that inhibition of PARP can prevent Tissue damage in myocardial and neurological ischemia, diabetes, septicemia, stroke and other diseases, and even contribute to chemotherapy and radiotherapy sensitization in tumor treatment. Research on PARP inhibitors has received extensive attention from researchers at home and abroad!
Amy Jie recommends a universal PARP assay kit for testing from Trevigen, USA. This universal kit detects the polymerization of biotinylated poly(ADP-ribose) onto a nearby histone in a 96-well plate. This kit is ideal for determining the known or possible PARP inhibitor IC50. For ease of operation, Trevigen also offers histone-coated strip holes, a design that saves analysis time. The universal PARP assay kit (containing histone-coated detachable plates) uses a colorimetric and chemiluminescent detection protocol.
PARP Analysis Kit Features:
1. Chemiluminescence/colorimetry, no radioactivity.
2. High-throughput 96-well plates with detachable plates for utilization.
3. High sensitivity, able to detect PARP in 10mU units.
4. The entire operation analysis process takes only 3 hours.
Amy Jie provides you with a one-stop PARP-related experimental solution, in addition to the universal PAPR assay kit (containing histone-coated detachable plates), as well as the PARP in vivo pharmacokinetic second-generation assay kit (PDAII) and HT Universal PARP Apoptosis Colorimetric Assay Kit, as well as related antibodies and recombinant proteins.
Trevigen is a fast-growing US biotechnology company focused on oncology research products and services for apoptosis, DNA damage and repair, tumor cell function and behavior. As the general agent of Trevigen in China, Amy Jie and Trevigen provide Chinese researchers with the best and latest quality products and technical services in the fields of oxidative stress, cell damage and tumor cell behavior research. If you are interested in the above products and solutions, please contact Ai Meijie Technology Co., Ltd. for an experimental solution for angiogenesis research, or request the latest product information.
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