TNF-α (tumor necrosis factor alpha) is a cytokine produced by macrophage/monocyte activation, a type II transmembrane protein consisting of 236 amino acids, in inflammatory response, cellular immunity, tumor immunity, etc. Play a key role in physiological and pathological processes. Rat cells expressing TNF-α are known to include B cells, colonic columnar epithelial cells, NK and CD3 CD56 liver natural T cells, macrophages, monocytes and monocyte-derived dendritic cells, CD4 and CD8 T Cells, mast cells, neutrophils, keratinocytes, plasma cells and fat cells.
When buying a rat TNF-α ELISA kit, these can't be ignored!
The EliKineTM Rat Tumor Necrosis Factor-α ELISA Kit quantifies the amount of TNF-α in a sample based on a two-site double-antibody sandwich ELISA. Antibodies specific for TNF-[alpha] have been pre-coated on 96-well plates. The target protein in the standard and the sample is bound to the immobilized antibody, and after removing the unbound substrate, the target protein-specific biotinylated detection antibody is added, and after washing, the patented streptavidin is added to the reaction well. Homo-HRP conjugate. Washing removes all unbound streptavidin-HRP reagent and adds HRP substrate solution to produce a colored product with a color depth proportional to the amount of protein of interest.
| Product name and description | Item number | product manual |
| EliKineTM Rat TNF-α ELISA Kit | KET9007 | Details |
*EliKineTM Rat Tumor Necrosis Factor-α ELISA Kit has extremely high sensitivity and specificity. No significant cross-reactivity or background interference has been detected in the detection of analogs of the target protein.
TNF-α ELISA Kit Product Features:
- High sensitivity: 25 pg/mL- 2000 pg/mL
- Gold standard antibody pairs and protein standards, the experimental results are reproducible
- Colorimetric detection method, no radioactivity
- Optimized standard double-antibody sandwich ELISA assay with standard working time of 3-5 hours
- Applicable samples: cell culture supernatant, serum, plasma, other biological fluids
Excellent standard curve icon
Abbkine technicians have been researching, developing and producing high quality immunoassay reagents for many years. We provide outstanding products to scientists in the life sciences worldwide and become trusted and reliable suppliers to many of the world's leading research institutions and clinical diagnostic companies. When buying a rat TNF-α ELISA kit, these can't be ignored!
With its excellent quality, the Crown Antibody series has been loved by researchers since its launch, and Abbkine is also encouraged. Based on years of experience in antibody development, Abbkine has once again developed a highly specific and sensitive EliKineTM ELISA kit that covers high levels of specificity and sensitivity, covering cytokines, hormones and other proteins, for human and mouse. Samples such as rats meet your diverse research needs. When buying a rat TNF-α ELISA kit, these can't be ignored!
More validated ELISA kits are available, please contact:
Abbkine China General Agent Website:
Or a local authorized Abbkine agent.
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