How to accurately evaluate the relationship between HTRF cytokine detection and changes in its cellular activity?

How to accurately evaluate   HTRF   What is the relationship between cytokine detection and changes in its cellular activity?

Cytokines (Cytokine, CK) is a class of proteins or small peptides can pass information between cells having immunomodulatory and effector function. Among them, inflammatory cytokines as an important component, mainly endogenous polypeptides produced by the immune system cells with many powerful biological effects , can mediate a variety of immune responses. Among the many inflammatory cytokines, TNF-α plays a major role.   , IL-1β , IL-6 , IL-8 and so on.

At present, there are many methods for detecting cytokines. Common methods include immunological detection and molecular detection. Among them, immunological detection methods are the most widely used, including ELISA , immunospot, immunofluorescence, and RIA . The latest advantages of a homogeneous time-resolved fluorescence detection ( HTRF ) technique , such as fast, homogeneous, sensitive and anti-interference , are well recognized. This method is also very suitable for monitoring cytokines and chemotaxis. The release of factors during cellular detection. In general, modulation of cytokine release relies on the use of pharmaceutical compounds to induce or inhibit their secretion. To further understand the mechanism of action of these drugs, we need to carefully analyze their effects, especially on the viability of cells.

This article describes how to better utilize integration   Molecular Devices Minimax300   Cell imaging module   SpectraMax i3x   Multi-functional microplate assay platform for analysis of cytokine secretion and cell viability. The method allows cytokine and cell activity assessments to be performed on a per-well / cell-by-cell basis, and the true effect of the drug is determined and normalized by cell morphology.