Introduction of anticoagulants commonly used in animal experiments
Physical or chemical methods are used to remove or inhibit certain clotting factors in the blood, preventing blood from clotting and becoming anticoagulated. Chemical agents that block blood clotting are called anticoagulants. The general requirement for anti-coagulants is that the amount is small, the solubility is large, and the measurement is not affected. The anticoagulants commonly used in biochemical tests are as follows:
1. Heparin: Heparin is a mucopolysaccharide containing a sulfate group. Its anticoagulant mechanism is mainly to prevent the formation and activity of thromboplastin and thrombin, and prevent platelet aggregation. Heparin anticoagulation is suitable for erythrocyte fragility test, blood gas analysis, hematocrit test, erythrocyte sedimentation rate and common biochemical assay; it is not suitable for hemagglutination test and white blood cell count. 0.5 ml of a 1.0 g/L heparin solution was used to anticoagulate 5 ml of blood. The anticoagulant concentration of heparin is 20~30 U/ml whole blood.
2. Potassium oxalate-sodium fluoride: Potassium oxalate can form calcium oxalate with calcium ions in the blood to prevent blood from coagulating. Potassium oxalate has a high solubility and strong anticoagulant effect. Fluoride ions can bind to calcium and resist coagulation, but the anticoagulant effect is weak. Fluoride ions inhibit the enolase in glycolysis and prevent glycolysis. If sodium fluoride is not added, the glucose content in the blood sample will degrade at a rate of 6% per hour. In the presence of sodium fluoride, the blood glucose concentration is stable for 24 hours at 25 ° C and 48 hours at 4 ° C. Therefore, potassium oxalate-sodium fluoride is a commonly used anticoagulant for blood glucose measurement specimens.
3. Ethylenediaminetetraacetic acid disodium (EDTA): EDTA and its salt are aminopolycarboxylic acids, which can effectively chelate calcium ions in blood samples. Chelation of calcium or removal of calcium from the reaction site will block and terminate the endogenous or exogenous coagulation process, thereby preventing blood samples from coagulating. Suitable for general hematology tests. Not suitable for blood coagulation test and platelet function test, nor for determination of calcium, potassium, sodium, iron, alkaline phosphatase, creatine kinase and leucine peptidase and PCR test. The anticoagulant concentration of EDTA is 3.4~4.8mmol/L whole blood.
4, sodium citrate: sodium citrate can form a complex with calcium ions in the blood, thereby preventing blood coagulation. The usual concentration is 109 mmol/L or 32.06 g/L.
Medical Cold Patch
Patch for diarrhea
[Name] Medical Cold Patch
[Package Dimension] 5cm 4pieces/box
The pain relief patch is composed of three layers, namely, backing lining, middle gel and protective film. It is free from pharmacological, immunological or metabolic ingredients.
[Scope of Application] For cold physiotherapy, closed soft tissue only.
[Indications]
The patches give a fast relief for diarrhea.
[How To Use a Patch]
Please follow the Schematic Diagram. One piece, one time.
The curing effect of each piece can last for 6-8 hours.
[Attention]
Do not apply the patch on the problematic skin, such as wounds, eczema, dermatitis,or in the eyes. People allergic to herbs and the pregnant are advised not to use the medication. If swelling or irritation occurs, please stop using and if any of these effects persist or worsen.notify your doctor or pharmacist promptly. Children using the patch must be supervised by adults.
[Storage Conditions]
Store below 30c in a dry place away from heat and direct sunlight.
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