Introduction:
In recent years, great progress has been made in the technique of large-scale cultivation of tumor cells in vitro to mimic the pathological environment in vivo. If the cultured tumor cells are added to the micropores of the round bottom, these enriched cells form discrete spheres. These discrete spheres contain both surface-exposed and deeply buried cells, proliferating and non-proliferating cells, an outer oxygen-rich layer and an internal anoxic center. Based on the above characteristics, compared with the traditional two-dimensional cell culture method, the sphere can better simulate the behavioral characteristics of the tumor. This three-dimensional tumor cell spheroid model has been successfully applied to the screening of tumor treatment methods. There are still some challenges in establishing a more mature and robust sphere experiment:
- Position the sphere in each well to image it in a single field of view
- Optimize the dyeing process to avoid the interference of the sphere while ensuring the permeability of the dye
- Get a clear image of the entire 3D structure, minimizing the effects of out-of-plane background signals and defocusing
- You can quickly analyze images to get meaningful results and draw charts at the same time
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