The culture medium of the strain determines the quality of the strain to a certain extent, and determines whether the production plan can be implemented smoothly. The choice of culture medium for producing strains should be based on local conditions and local materials, such as cottonseed husks and corncobs in the northern regions. Rice husks, rice bran, and lotus seed husks in the south can be applied. There are also wheat, sawdust, and other resources available everywhere. It is also a good base material. However, in long-term research and development practices, it has been found that many producers or dogmatists, or biased listeners, blindly plan and use resources that are not available locally, leading to increased production costs. The actual effect is not satisfactory.
One-grade medium formulation:
PDA (potato dextrose agar) modified medium is generally used. Basic formula: 200 grams of potato, 30 kg of wheat bran, 20 grams of glucose, 3 grams of potassium dihydrogen phosphate, 2 grams of magnesium sulfate. 4 mother eaters. Vitamin B11 tablets. The formula is suitable for most varieties, and it is usually about 8 days.
Secondary seed matrix and its formulation:
1. Cottonseed husk medium formulation: 95kg cottonseed husk, 5kg wheat bran, 2kg lime powder. Superphosphate 2 kg, urea 0.3 kg. It is suitable for most varieties such as Pleurotus ostreatus, straw mushroom, Coprinus comatus, Pleurotus eryngii, Pleurotus eryngii, Pleurotus eryngii, and Bailing Mushroom. General germination about 25 days.
2. Grain medium formulation: 100 kg of wheat, 20 kg of wheat bran, 1 kg of lime powder, and 1.5 kg of gypsum powder. Grains such as corn, soybeans, etc. can also be used. The formula is suitable for most species such as Pleurotus ostreatus, Pleurotus eryngii, Pleurotus citrinopileatus, Coprinus comatus, Enoki mushroom, Pleurotus eryngii, and Lingzhi mushroom. General germination about 10 days.
3, sawdust medium formula: wood chips 80 kg, wheat bran 15 kg. Bean cake powder 5 kg, lime powder 5 kg, gypsum powder 2 kg, superphosphate 2 kg, urea 0.6 kg. The formula is suitable for most kinds of wood rot fungi such as Pleurotus ostreatus, Pleurotus ostreatus, Pleurotus citrinopileatus, Flammulina velutipes, Pleurotus eryngii and Pleurotus eryngii. General germination about 40 days.
4. The basic formulation of corn stover and other straw substrates: 85 kg of corn cob, 15 kg of wheat bran, 4 kg of lime powder, and 2 kg of gypsum powder. Superphosphate 2 kg, urea 0.6 kg. The formula is suitable for most species such as Pleurotus ostreatus, straw mushroom, Coprinus comatus, Pleurotus ostreatus, Pleurotus citrinopileatus, Flammulina velutipes, Pleurotus eryngii, Pleurotus nebrodensis and the like General germination about 10 days.
5. Basic formula of wood matrix: 100 kg of wood or wood, 20 kg of wheat bran, 4 kg of lime powder, 2 kg of gypsum powder, 2 kg of superphosphate, and 1 kg of urea. The formula is suitable for mushrooms and other varieties, minus the lime powder can be applied to the fungus class. General germination about 20 days.
Tertiary seed matrix and formulation:
Can be based on secondary species. According to the principle of cost reduction, corn cobs can be used as the main material, and high-value raw materials such as wheat bran can be subtracted.
Adjust the matrix as required
1. When using black cotton fungus, hair fungus, Hericium erinaceus and other varieties, when using cotton seed shell matrix, lime powder should be reduced or removed on the basis of basic formula.
2. When the bacteria need to be used as quickly as possible, the following methods may be taken:
The plan must be planted for 20 days, increasing the number of production of one species. The seeding plan can be completed by simultaneously increasing the secondary seed quantity and using the grain matrix. When the specific number of bacteria, raw material or fermentation material is cultivated, 50-80 secondary species can be calculated for each hundred kilograms of raw materials, and the number of primary species can be calculated simultaneously.
The plan must be planted in 40 days, increase the amount of production of one species, increase the number of secondary species and use the grain matrix simultaneously; the third-grade species can use small-species bacteria seed bags, cottonseed husk culture medium to ensure that the bacteria can be finished within 20 days. Satisfy sowing plan. The calculation method is similar to the above.
The plan must be planted for 60 days, and the first-class kind of conventional production; the second and third-class seeds should be produced in a conventional manner using cottonseed husks, corn cobs, and other media; the planting plan can be easily satisfied. The calculation method is the same as above.
Several special conditions to pay attention to the problem:
Straw mushroom: The secondary species has secondary pollution characteristics and should be used promptly; the tertiary species can quickly mushroom. Not long-term survival.
Coprinus comatus: strains will not bud, can be stored for a period of time, but should pay attention to save the environment. Prevent pests and high temperatures.
Agaricus bisporus: Especially high-temperature strains, once a large primordium occurs around the material surface, it should not be used as a strain.
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