purpose:
Mass milling of corn leaves provides convenience for subsequent extraction of nucleic acids for genetic or physiological biochemical analysis. For the seed and agricultural product quality management department, seed companies and other units to use molecular methods to detect sample purity, detect the authenticity of purity and other sample pre-processing work necessary for reference.
Location: A certain industry breeding and cultivation laboratory
Material: fresh corn seedling leaves
Instrument: Mainframe, TL2020 automatic freeze grinder (also known as TL2020 high-throughput tissue grinder); Accessories: 2ml grinding adapter , 3 or 5mm diameter grinding beads
step:
1 ) Sampling: Take the leaves of fresh corn seedlings and cut them into pieces of 6~8mm in diameter ;
2) The 3-5 fragment of blades disposed 2ml centrifuge tube (EP tube), while adding a 3mm 5mm and two stainless steel ball milling.
3 ) Seal the EP tube that is added to the blade and the grinding steel ball , put it into the 2ml grinding adapter of the TL2020 automatic freezing grinder , and then freeze it with the adapter into the liquid nitrogen for 2-3 minutes or until the liquid nitrogen does not “boilingâ€. until.
4 ) Quickly remove the adapter from the liquid nitrogen using the DHS- specific frozen basket and quickly fix it in the rocker arm of the grinder;
5 ) Set the grinding time of TL2020 to 2 minutes and the grinding frequency to 1500 rpm ( 1500 times / min).
result:
Very finely divided leaf powder can be obtained, and the extraction of nucleic acids works well.
in conclusion:
The TL2020 automatic freeze grinder can be used for freeze-grinding corn leaves. The grinding tube and adapter can be directly immersed in liquid nitrogen. It can be batched in 1-2 minutes with liquid nitrogen freezing and grinding for about 2 minutes. Crush up to 54 samples of 2.0 ml ( 192 samples with 96- well deep well plates ). The treatment time is fast, effectively preventing the temperature rise, and providing a guarantee for the subsequent effective early nucleic acid. In addition, it also saves a lot of liquid nitrogen and is safer to operate.
Keywords: freeze grinding, freeze grinding machine, automatic freezing grinder
Tazarotene Intermediate,Tazarotene Api,Tazarotene Cas 118292-40-3,Tazarotene Intermediates
Shandong Bolode Bio-technology Co., Ltd. , https://www.bldpharma.com