At present, the instrument structure of the ultraviolet-visible spectrophotometer is generally divided into three types: single beam, double beam and dual wavelength.
1. Single beam UV-visible spectrophotometer
A single beam refers to the light emitted from the source, through a monochromator, etc.—a series of optical components that pass through the absorption cell and are always illuminated as a beam of light when illuminated on the detector. It only has a bundle of monochromatic light (the beam can only alternately pass through the reference solution, the sample solution), a cuvette, and a photoelectric converter. When working, a light path, first through the reference solution, and then through the sample solution for light intensity measurement.
The single-beam UV-visible spectrophotometer is characterized by simple structure and low price, and is mainly suitable for quantitative analysis. The measurement result is greatly affected by the fluctuation of the power supply, and it is easy to bring a large error to the measurement result. Therefore, in general, the single-beam UV-Vis spectrophotometer is not suitable for the pharmaceutical industry and the quality inspection industry.
Second, dual beam UV-visible spectrophotometer
A dual-beam UV-visible spectrophotometer is an ultraviolet-visible spectrophotometer with two monochromatic lights. Its optical path design is basically similar to a single beam spectrophotometer. The difference is that a cutter is added between the monochromator and the absorption cell. The function is to divide one beam into two beams of equal intensity at a certain frequency, so that one pass through the reference solution and the other through the sample solution. The reference signal and the sample signal are then alternately received by the detector. After the received optical signal is converted into an electrical signal, it is amplified by a preamplifier, further demodulated, amplified, compensated, etc., and finally displayed by the display system.
Common double-beam UV-visible spectrophotometric juices are mainly 710, 730, 760cRT and so on.
Shanghai Instrument Analysis 760CRT Dual Beam UV-Vis Spectrophotometer
Three-wavelength UV-visible spectrophotometer
The dual-wavelength UV-Vis spectrophotometer uses two monochromators to obtain two monochromatic lights of different wavelengths at the same time. The light emitted by the optical path source is separated by two monochromators to produce a single color with a wavelength of 4: and 4z. Light, through the dimmer, alternately illuminate the two beams of light at a certain time interval to the same absorption cell containing the sample solution, and the detector displays the transmittance or absorbance of the test solution at wavelengths 4 and 42.
The dual-wavelength spectrophotometer can not only measure high-concentration samples, multi-component mixed samples, but also is more sensitive and selective than single-wavelength measurement when measuring turbid samples.
(Content source Hengtianli Instrument Network)
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